A simple, sensitive, rapid and precise bioanalytical RP-HPLC method was developed for estimation of albendazole in rat plasma. The work carried out on Shimadzu LC-2010 CHT HPLC system equipped with Purospher® STAR Hibar® 250 mm C18 (4.6 mm × 25 cm, packed with 5 m particles) column with mobile phase containing sodium acetate buffer (pH 4.7): acetonitrile in the ratio 50:50 v/v (1 ml/min) and detection wavelength 298 nm. The retention time of albendazole was found to be 5.5 min. The developed bioanalytical method was found to be linear in concentration range of 0.43-2.60 ng/ml (R2 = 0.9466). The precision study revealed that the percentage cumulative variation was within acceptable limit and accuracy study showed the value of mean percent recovery between 89.73-108.99 %. The albendazole was stable in rat plasma at different storage conditions. The validation parameters of the method met the acceptance criteria. Sufficient stability at LQC and HQC was shown to allow for completion of sample analysis in clinical trials. From the results, it was concluded that developed bioanalytical method can be used for routine analysis of albendazole.
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